Inhibition by Naloxone of Promoter Activity of the Neurofilament Gene in SK-N-SH Cells

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Chronic administration of morphine is known to decrease the levels of neurofilaments(NFs)in the ventral tegmental area.We ligated a promoter region of the mouse 68−KDa neurofilament(NF−68)gene to the pGL3−enhancer vector containing a luciferase gene, transfected it into SK−N−SH cells and then analyzed transcriptional activity in the cells treated with agonists or antagonists of opiate receptors.The activity of the NF−68 promoter was suppressed by naloxone about 55% at 10<SUP>-5</SUP> M and 30% at 10<SUP>-7</SUP> M at 48h, but suppressed not by morphine.Naltrexone at 10<SUP>-5</SUP> M suppressed the promoter activity about 20%, but levallorphan, DAMGO, DPDPE and U50488 did not.The inhibition by naloxone was dose−dependent and not reversed by morphine.The inhibitory effect of naloxone was not observed in N18TG−2 cells and PC12 cells.Experiments with various deletion mutants revealed that a region responsible for naloxone suppression spans from −328 to − 101 in the gene.These results suggest that naloxone has the ability to suppress transcriptional activity in some neurons.

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  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 82(1), 34-39, 2000-01-01

    公益社団法人 日本薬理学会

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各種コード

  • NII論文ID(NAID)
    10008182601
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4966438
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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