Characterization of Protease-Activated Receptors in Rat Peritoneal Mast Cells

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Author(s)

Abstract

Activation of protease−activated receptor(PAR)−1 or PAR−2 elicits inflammation most probably via mast cell degranulation in vivo.The present study aimed at characterizing PARs in rat peritoneal mast cells(PMC).Messenger RNA for PAR−1, but not for PAR−2, was detected in PMC.Thrombin, the PAR−1 agonist SFLLR−NH<SUB>2</SUB> or the PAR−2 agonist SLIGRL−NH<SUB>2</SUB> failed to induce histamine release from PMC.Surprisingly, the PAR−2−inactive control peptide LSIGRL−NH<SUB>2</SUB> triggered histamine release from PMC.Thus, PAR−1, but not PAR−2, are expressed in PMC, whereas neither PAR−1 nor PAR−2 are considered to be involved in degranulation of PMC.LSIGRL−NH<SUB>2</SUB> does not appear to be appropriate as a control peptide for PAR−2 in inflammation studies.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 82(1), 74-77, 2000-01-01

    The Japanese Pharmacological Society

References:  15

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008182779
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4966505
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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