1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) Inhibits Cyclic GMP-PKG Pathway-Independent Nonadrenergic, Noncholinergic Relaxation in Longitudinal Muscle of the Rectum of Wistar-ST Rats

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Author(s)

    • Nakagawa Masashi NAKAGAWA Masashi
    • Department of Veterinary Pharmacology, College of Agriculture, Research Institute for Advanced Science and Technology, Osaka Prefecture University
    • Niioka Satomi [他] NIIOKA Satomi
    • Department of Veterinary Pharmacology, College of Agriculture, Research Institute for Advanced Science and Technology, Osaka Prefecture University
    • YAMAJI Michiru
    • Department of Veterinary Pharmacology, College of Agriculture, Research Institute for Advanced Science and Technology, Osaka Prefecture University
    • OKISHIO Yutaka
    • Department of Veterinary Pharmacology, College of Agriculture, Research Institute for Advanced Science and Technology, Osaka Prefecture University
    • NISHIO Hideaki
    • Department of Veterinary Pharmacology, College of Agriculture, Research Institute for Advanced Science and Technology, Osaka Prefecture University
    • HATA Fumiaki
    • Department of Veterinary Pharmacology, College of Agriculture and Department of Molecular Physiology and Biochemistry, Research Institute for Advanced Science and Technology, Osaka Prefecture University

Abstract

Participation of the nitric oxide-cyclic GMP pathway in nonadrenergic, noncholinergic(NANC)relaxation induced by electrical field stimulation of longitudinal muscle of the rectum of Wistar-ST rats was studied by using a selective inhibitor of soluble guanylyl cyclase, 1H-[1, 2, 4]oxadiazolo[4, 3-a]quinoxalin-1-one(ODQ).ODQ concentration dependently inhibited the relaxation and at 10μM, maximally inhibited it by 83%.However, results obtained with N<SUP>G</SUP>-nitro-L-arginine, L-arginine and exogenously added nitric oxide excluded the participation of nitric oxide in the relaxation.An inhibitor of cyclic GMP-dependent protein kinase(PKG)partially(39%)inhibited the relaxation.ODQ also significantly inhibited the relaxation, which persisted after the PKG inhibitor-treatment, by 85%.The results strongly suggest that ODQ inhibits the NANC relaxation in a cyclic GMP-PKG pathway-independent manner.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 82(2), 164-167, 2000-02-01

    The Japanese Pharmacological Society

References:  15

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008183237
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5284002
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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