Purinoceptor-Mediated Calcium Mobilization and Cellular Proliferation in Cultured Bovine Corneal Endothelial Cells

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Author(s)

Abstract

In the present study, we investigated the effect of adenosine triphosphate(ATP)on cytosolic free calcium mobilization and mitogenic activity in cultured bovine corneal endothelial cells(BCEC).The[Ca<SUP>2+</SUP>]<SUB>i</SUB> was determined using a Ca<SUP>2+</SUP> sensitive indicator, Fura-2/AM, and cell proliferation was evaluated by counting the cell number.ATP, its metabolites and analogs caused transient increase in[Ca<SUP>2+</SUP>]<SUB>i</SUB> in a concentration-dependent manner(10<SUP>-7</SUP>M−10<SUP>-3</SUP>M)and the potency of agonists was ordered as follows:2-methylthio-ATP>uridine triphosphate>ATP>adenosine diphosphate.Adenosine monophosphate and adenosine did not affect[Ca<SUP>2+</SUP>]<SUB>i</SUB>.ATP(10<SUP>-4</SUP>M)also promoted the accumulation of inositol trisphosphate(IP<SUB>3</SUB>).The ATP-induced transient[Ca<SUP>2+</SUP>]<SUB>i</SUB> increase and IP<SUB>3</SUB> accumulation were attenuated by pretreatment with a phospholipase C inhibitor, U-73122(5μM), for 30 min.ATP(10<SUP>-5</SUP>M)significantly enhanced the proliferation of BCEC.ATP-induced[Ca<SUP>2+</SUP>]<SUB>i</SUB> increase and cell proliferation were inhibited by a purinoceptor antagonist, suramin(10<SUP>-4</SUP>M).Thus, the present study indicates that BCEC contain P2 purinoceptors that regulate their proliferation.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 82(3), 181-187, 2000-03-01

    The Japanese Pharmacological Society

References:  29

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008183354
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5320972
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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