Protein Kinase C Potentiates Capacitative Ca^<2+> Entry That Links to Steroidogenesis in Bovine Adrenocortical Cells

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Author(s)

Abstract

I investigated the role of protein kinase C(PKC) in regulation of the capacitative Ca<SUP>2+</SUP> entry and steroidogenesis in bovine adrenocortical(BA)cells.Thapsigargin(TG)-treatment depleted intracellular Ca<SUP>2+</SUP> stores followed by induction of Ca<SUP>2+</SUP> influx from the extracellular pool and also increasing of Mn<SUP>2+</SUP> influx as an indicator of divalent cation influx in BA cells.Calphostin C, a PKC inhibitor, inhibited the TG-induced [Ca<SUP>2+</SUP>]<SUB>i</SUB> elevation dose-dependently(0.1-1μM)and attenuated Mn<SUP>2+</SUP> entry.Phorbol 12-myristate 13-acetate(PMA), an activator of PKC, potentiated the elevation of[Ca<SUP>2+</SUP>]<SUB>i</SUB> and enhanced Mn<SUP>2+</SUP> entry by TG treatment.These results suggest that PKC may modulate capacitative Ca<SUP>2+</SUP> entry in BA cells.In the presence of extracellular Ca<SUP>2+</SUP>, TG enhanced cortisol production in BA cells.Calphostin C attenuated the TG-induced steroidogenesis dose-dependently(0.25-1μM).PMA enhanced the steroidogenesis dose-dependently(1-100nM).These results suggested that PKC may have a modulatory effect on the capacitative Ca<SUP>2+</SUP> entry that links to steroidogenesis in BA cells.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 82(3), 210-217, 2000-03-01

    The Japanese Pharmacological Society

References:  31

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008183462
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5320985
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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