Evaluation of Neurotoxicity of Alzheimer's Amyloid β protein (β42) in Cultured Hippocampal Cells and Its Prevention by Propentofylline

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Abstract

Neurotoxicity of β42(20μM) in cultured rat hippocampal neurons was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) reduction and lactate dehydrogenase(LDH) release methods as quantitative assays of cell death, and both methods indicated that propentofylline(PPF) had the ability to protect the neurons against the toxicity, although these two assay methods revealed different mechanisms for the toxic effect of β42.Promotion of the active exocytotic system of the cells was suggested after treatment with β42 in the MTT assay and in determination of 9-aminoacridine(AA) excretion from the preloaded cells after 24-h treatment with β42.The promotion of AA exocytosis was blocked by the addition of PPF(20μg/ml).The preventive effect of PPF on the neurotoxicity of β42 has been proposed to be caused by elevation of the intracellular level of cAMP as a result of depression of the hydrolytic activity of cells.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 82(4), 301-306, 2000-04-01

    The Japanese Pharmacological Society

References:  27

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008183832
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5362740
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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