New Approach for Measurement of Sympathetic Nervous Abnormality in Conscious, Spontaneously Hypertensive Rats

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Author(s)

    • INA Yasuhiro
    • Drug Development Research Laboratories, Pharmaceutical Research, Institute, Kyowa Hakko Kogyo Co., Ltd
    • NAGASHIMA Ken
    • Drug Development Research Laboratories, Pharmaceutical Research, Institute, Kyowa Hakko Kogyo Co., Ltd
    • OHMORI Kenji
    • Drug Development Research Laboratories, Pharmaceutical Research, Institute, Kyowa Hakko Kogyo Co., Ltd
    • KOBAYASHI Satoshi
    • Drug Development Research Laboratories, Pharmaceutical Research, Institute, Kyowa Hakko Kogyo Co., Ltd
    • IMAI Kazuhiro
    • Department of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo

Abstract

We previously reported a highly sensitive chemiluminescence high−performance liquid chromatographic method to determine catecholamines in plasma.In this study, we employed this method to measure the cardiac function and plasma norepinephrine(NE)concentration in conscious rats.Benidipine, 1, 4−dihydropyridine calcium antagonist(4 mg/kg), and β−blocker(propranolol, 30 mg/kg)were administered orally to conscious spontaneously hypertensive rats(SHRs)and Wistar−Kyoto(WKY)rats, and blood pressure, heart rate and plasma NE levels were measured.Plasma NE concentration was used as an index of sympathetic nervous system activity in conscious rats.The basal plasma NE levels were significantly higher in SHRs than in WKY rats(P<0.05), indicating the activity of the basal sympathetic nervous system in SHRs was elevated.The sensitivity of the baroreflex−mediated sympathetic nervous response was reduced in SHRs as compared to that in WKY rats.The concomitant administration of benidipine and a β−blocker decreased heart rate without affecting the baroreflex−mediated sympathetic nervous response, indicating that propranolol might suppress mainly the cardiac β−adrenoceptor.The present study suggested the high activity of the basal sympathetic nervous system and the reduced response of the baroreflex−mediated sympathetic nervous system in SHRs compared to WKY rats in the conscious condition.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 83(1), 39-45, 2000-05-01

    The Japanese Pharmacological Society

References:  29

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008184318
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5399697
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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