Regulatory Machanism of Eosinophil Peroxidase Release From Guinea Pig Eosinophils

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Author(s)

Abstract

The regulatory mechanism of degranulation of guinea pig peritoneal eosinophils was studied by determination of eosinophil peroxidase(EPO)release.β−Agonists, such as isoproterenol, salbutamol and fenoterol, effectively inhibited A23187−induced EPO release from guinea pig eosinophils.The inhibitory effects of β−agonists were attenuated by pretreatment with either propranolol, a non−selective β−antagonist, or ICI 118, 551, a selective β<SUB>2</SUB>−antagonist.Both theophylline and dibutyryl−cAMP(db−cAMP)also significantly inhibited A23187−induced EPO release.The inhibition of EPO release induced by db−cAMP was attenuated by pretreatment with KT5720, a protein kinase A inhibitor.In addition, calphostin C as well as cytochalasin D effectively inhibited A23187−induced EPO release.From the results of the present study, it was concluded that an increase in intracellular Ca<SUP>2+</SUP> concentration may lead to exocytosis of eosinophil granules through activation of protein kinase C and microfilaments.β−Agonists and theophylline were effective in inhibiting degranulation of eosinophils by increasing intracellular cAMP level coupled with the activation of protein kinase A.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 83(4), 293-299, 2000-08-01

    The Japanese Pharmacological Society

References:  24

Codes

  • NII Article ID (NAID)
    10008184599
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    5501173
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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