Influences of Everninomicin, Vancomycin and Teicoplanin on Chemical Mediator Release From Rat Peritoneal Mast Cells

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Author(s)

Abstract

We examined influences of certain antibiotics on the release of chemical mediators from isolated rat peritoneal mast cells in vitro.Isolated peritoneal mast cells were obtained from male Wistar strain rats.Everninomicin(0.06−1.2 mg/ml), vancomycin(0.05−1.0 mg/ml), teicoplanin(0.07−1.4 mg/ml)and concanavalin A(0.01 mg/ml)were used.Isolated mast cells were incubated in the presence of various concentrations of the test compound at 37°C for 10 min.Histamine contents of the supernatant and cell pellet were measured by an automated fluorometric method.Prostaglandin D<SUB>2</SUB>(PGD<SUB>2</SUB>)contents of the supernatant were determined by enzyme immunoassay.Everninomicin(0.06−1.2 mg/ml)had no influence on histamine and PGD<SUB>2</SUB> release from mast cells.On the other hand, vancomycin significantly released both histamine(0.5 and 1.0 mg/ml)and PGD<SUB>2</SUB>(1.0 mg/ml)from mast cells, but vancomycin did not affect concanavalin A−induced histamine and PGD<SUB>2</SUB> release.Teicoplanin(more than 0.07 mg/ml)significantly stimulated histamine and PGD<SUB>2</SUB> release from mast cells and it also significantly potentiated concanavalin A−induced histamine and PGD<SUB>2</SUB> release.These results suggest that everninomicin causes no chemical mediator release from mast cells, different from vancomycin and teicoplanin.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 83(4), 300-305, 2000-08-01

    The Japanese Pharmacological Society

References:  30

Codes

  • NII Article ID (NAID)
    10008184624
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    5501181
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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