Ca^<2+> Buffering Function of Sarcoplasmic Reticulum in Rat Tail Arteries: Comparison in Normotensive and Spontaneously Hypertensive Rats

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Author(s)

Abstract

The superficial buffer barrier function of the sarcoplasmic reticulum(SR)during rest and that during stimulation with Bay k 8644, an agonist of L−type Ca<SUP>2+</SUP> channels, were compared in endotheliumdenuded strips of tail arteries from 13−week−old normotensive Wistar−Kyoto rats(WKY)and spontaneously hypertensive rats(SHR), by measuring the effects of cyclopiazonic acid(CPA)and thapsigargin that inhibit SR Ca<SUP>2+</SUP>−ATPase and the effect of ryanodine that depletes SR Ca<SUP>2+</SUP>.The addition of 10μM CPA induced a transient contraction that was not significantly different between WKY and SHR.The CPA−induced contraction was strongly inhibited by 100 nM nifedipine and was abolished by Ca<SUP>2+</SUP>−free solution in both strains.Thapsigargin(100 nM)or ryanodine(10 μM)induced similar, small transient contractions in the two strains.The addition of Bay k 8644(1−100 nM)almost failed to induce a contraction in both WKY and SHR.When the strips were preincubated with 10 μM CPA, 100 nM thapsigargin or 10 μM ryanodine, Bay k 8644 induced similar concentration−dependent contractions in the two strains.The amount of Ca<SUP>2+</SUP> stored in the SR, as estimated from the 20 mM caffeine−induced contraction, was not significantly different between WKY and SHR.Our results suggest that the SR of rat tail arteries can buffer a large amount of Ca<SUP>2+</SUP> that enters the cell during the rest and the Bay k 8644 stimulation, and these functions are not altered in SHR.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 83(4), 335-343, 2000-08-01

    The Japanese Pharmacological Society

References:  26

Codes

  • NII Article ID (NAID)
    10008184790
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    5501279
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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