Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein

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Author(s)

    • Kimura Yuji KIMURA Yuji
    • Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
    • WAKAMATSU Daisuke
    • Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
    • KOZAKI Ryo-hei
    • Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
    • HASHIMOTO Hitoshi
    • Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
    • MATSUDA Toshio
    • Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
    • BABA Akemichi
    • Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University

Abstract

Expression of Bcl−2 related proteins in rat microglial primary culture was examined.At relative low cell densities, serum deprivation caused cell death and nuclei condensation of cultured microglia.Expression of a pro−apoptotic protein, Bax, but not Bcl−2, was increased by the serum deprivation.SB203580, a specific inhibitor of p38<SUP>MAPK</SUP>, prevented both the serum deprivation−induced Bax expression and microglial death.Immunochemical staining showed that microglia expressing a high level of Bax were subjected to apoptosis−like cell death.These observations suggest that Bax expression underlies the apoptosis of cultured microglia after serum deprivation.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 83(4), 351-354, 2000-08-01

    The Japanese Pharmacological Society

References:  14

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008184843
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5501315
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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