Serotonin Induces Apoptosis in PGT-β Pineal Gland Tumor Cells

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Author(s)

Abstract

Serotonin−induced neuronal cell death has been implicated as a possible cause of neurodegenerative and neuropsychiatric disorders.To investigate the involvement of serotonin−induced apoptosis as a potential mechanism in the pathophysiology of serotonin−related diseases, MTT(3−(4, 5−dimethylthiazol−2−yl)−2, 5−diphenyltetrazolium bromide)assay, DAPI(4’, 6−diamidino−2−phenylindole)staining, TUNEL(terminal deoxynucleotidyl transferase−mediated dUTP−biotin nick end labeling)assay and flow cytometric analysis were performed using the immortalized pineal cell line PGT−β.Through morphological and biochemical analyses, it was demonstrated that cell death induced by serotonin in PGT−β cells shows classic apoptotic features.These data suggest that serotonin induces apoptosis in PGT−β cells.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 84(1), 71-74, 2000-09-01

    The Japanese Pharmacological Society

References:  15

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008185219
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5526094
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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