Intracellular Ca^<2+> Increase in Neuro-2A Cells and Rat Astrocytes Following Stimulation of Bradykinin B_2 Receptor

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Author(s)

Abstract

Murine neuroblastoma cell line Neuro−2A cells and rat brain astrocytes showed a dose−dependent increase in intracellular Ca<SUP>2+</SUP> in response to bradykinin, when assessed by a single cell image analyzing system.The Ca<SUP>2+</SUP> increase in Neuro−2A cells by bradykinin was also examined by a suspension fluorescent assay using fura−2 loading.The Ca<SUP>2+</SUP> increase in both cases was suppressed by a bradykinin B<SUB>2</SUB> receptor antagonist, Hoe 140, but not by a B<SUB>1</SUB> receptor antagonist, des−Arg−Hoe 140, suggesting that the effect occurred via specific B<SUB>2</SUB> receptor activation.RT−PCR for bradykinin B<SUB>2</SUB> receptor mRNA showed that both Neuro−2A cells and the astrocytes expressed B<SUB>2</SUB> receptor mRNA.Binding of [<SUP>3</SUP>H]bradykinin to Neuro−2A cells was assessed, and a specific binding constant of 0.75 nM was determined.Furthermore, the increase in [Ca<SUP>2+</SUP>]<SUB>i</SUB> by bradykinin could be caused by a release of Ca<SUP>2+</SUP> from storage sites in the endoplasmic reticulum, since thapsigargin and U−73122 attenuated the effect of bradykinin in Neuro−2A as well as in astrocytes.These results indicate that both astrocytes and neuroblastoma Neuro−2A cells stimulated by bradykinin could express a bradykinin B<SUB>2</SUB> receptor−mediated intracellular Ca<SUP>2+</SUP> increase leading to signal transduction.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 84(2), 140-145, 2000-10-01

    The Japanese Pharmacological Society

References:  20

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008185550
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5549207
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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