Effect of Endothelin-1 (1-31) on Human Mesangial Cell Proliferation.
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- Yoshizumi Masanori
- Department of Pharmacology and The University of Tokushima School of Medicine,Tokushima 770-8503,Japan
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- Kagami Shoji
- Department of Pediatrics,The University of Tokushima School of Medicine,Tokushima 770-8503,Japan
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- Suzaki Yuki
- Department of Pharmacology and The University of Tokushima School of Medicine,Tokushima 770-8503,Japan
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- Tsuchiya Koichiro
- Department of Pharmacology and The University of Tokushima School of Medicine,Tokushima 770-8503,Japan
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- Houchi Hitoshi
- Department of Pharmacology and The University of Tokushima School of Medicine,Tokushima 770-8503,Japan
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- Hisayama Tetsuhiro
- Department of Pharmacology,Faculty of Pharmaceutical Sciences,The University of Tokushima,Tokushima 770-8505,Japan
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- Fukui Hiroyuki
- Department of Pharmacology,Faculty of Pharmaceutical Sciences,The University of Tokushima,Tokushima 770-8505,Japan
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- Tamaki Toshiaki
- Department of Pharmacology and The University of Tokushima School of Medicine,Tokushima 770-8503,Japan
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Abstract
It was previously found that human chymase cleaves big endothelins(ETs)at the Tyr31−Gly32 bond and produces 31−amino acid ETs(1−31).In the present study, human plasma concentrations of ET−1(1−31)and ET−1 were examined and the effect of synthetic ET−1(1−31)on the proliferation of cultured human mesangial cells(HMCs)was investigated.The proliferative effect of ET−1(1−31)was evaluated from the [3H]−thymidine uptake.The activity of extracellular signal−regulated kinase(ERK)and DNA binding activity of activator protein−1 were determined by using an in−gel kinase assay and gel mobility shift assay, respectively.Immunoreactive ET−1(1−31)was detectable in plasma, but the level was slightly lower than that of ET−1.ET−1(1−31)increased [3H]−thymidine incorporation in HMCs to a degree similar to that induced by ET−1.ET−1(1−31)also activated ERK1/2.Inhibition of protein kinase C and ERK kinase caused a reduction of ET−1(1−31)−induced ERK1/2 activation.The ERK1/2 activation was followed by an increase in transcription factor activator protein−1 DNA binding activity.These findings suggest that ET−1(1−31)is a bioactive peptide in humans and ET−1(1−31)itself stimulates HMC proliferation.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 84 (2), 146-155, 2000
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390001204287569408
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- NII Article ID
- 10008185571
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- NII Book ID
- AA00691188
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- COI
- 1:CAS:528:DC%2BD3cXnvVGgtro%3D
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- ISSN
- 13473506
- 00215198
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- NDL BIB ID
- 5549225
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- PubMed
- 11128037
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I5549225
- https://api.elsevier.com/content/article/PII:S0021519819305268?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819305268?httpAccept=text/plain
- https://www.jstage.jst.go.jp/article/jjp/84/2/84_2_146/_pdf
- https://search.jamas.or.jp/link/ui/2001048021
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed