Investigation Into the 5-Hydroxytryptophan-Evoked Luminal 5-Hydroxytryptamine Release From the Guinea Pig Colon

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The effect of an endogenous 5−hydroxytryptamine(5−HT)precursor, 5−hydroxytryptophan(5−HTP), on the luminal outflow of 5−HT was examined using the luminally perfused isolated colon of the guinea pig, a model that would facilitate the pharmacological analysis of luminal 5−HT release from enterochromaffin cells(EC cells).5−HTP(1−10μM)concentration−dependently caused an increase of the luminal outflow of 5−HT.Either tetrodotoxin(0.3μM)or atropine(0.2μM)did not affect the 5−HTP−evoked increase in luminal 5−HT outflow, while the L−type calcium channel blocker, nicardipine(1μM)or diltiazem(1μM)reduced the 5−HTP−evoked 5−HT outflow by 47% and 61%, respectively.SB203186(1μM), a 5−HT<SUB>4</SUB>−receptor antagonist, enhanced the 5−HTP−evoked 5−HT outflow, while ramosetron(1μM), a 5−HT<SUB>3</SUB>−receptor antagonist reduced the stimulating effect of 5−HTP by 66%.Ketanserin(0.1μM), a 5−HT<SUB>2A</SUB>−receptor antagonist did not modify the stimulatory effect of 5−HTP.It is concluded that in the guinea pig colon, 5−HTP facilitates the luminal 5−HT release from EC cells, with no involvement of neuronal mechanisms and a non−neuronal cholinergic system.Furthermore, non−neuronal 5−HT<SUB>3</SUB> and 5−HT<SUB>4</SUB> receptors appear to contribute to the regulation of the luminal 5−HT release evoked by 5−HTP.This new bioassay of the guinea pig colon allows the pharmacological characterization of uncomplicated luminal 5−HT release from EC cells.

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  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 84(2), 174-178, 2000-10-01

    公益社団法人 日本薬理学会

参考文献:  19件中 1-19件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10008185687
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    5549312
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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