Correlation Between Adenine Nucleotide-Induced Cyclic AMP Elevation and Extracellular Adenosine Formation in NG108-15 Cells

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Author(s)

Abstract

We previously demonstrated that extracellular adenine nucleotides induced cyclic AMP elevation in NG108-15 cells. This response was resistant to adenosine deaminase (ADA) and the ecto-5′-nucleotidase (CD73) inhibitor α, β-methylene ADP(α, β-MeADP), but was inhibited by both P1- and P2-receptor antagonists. In the present study, we investigated the relationship between adenine nucleotide-induced cyclic AMP elevation and extracellular adenosine formation. ATP, AMP and β, γ-methylene ATP (β, γ-MeATP) were time-dependently metabolized to adenosine in NG108-15 cells. Adenosine formations from ATP, AMP and β, γ-MeATP were not affected by α, β-MeADP, but suppressed by the P2-receptor antagonist pyridoxalphosphate-6-azophenyl-2′, 4′-disulphonic acid (PPADS). A close correlation between extracellular adenosine formation and cyclic AMP increasing effects were obtained with several adenine nucleotide agonists in NG108-15 cells as well as their parent cell line C6Bu-1 and N18TG-2 cells, all of which possess functional adenosine A<SUB>2</SUB> receptors. When NG108-15 cells were incubated with [<SUP>3</SUP>H]ATP or [<SUP>3</SUP>H]AMP in the presence of ADA, [<SUP>3</SUP>H]adenosine was found to distribute dominantly on the cell surface. NG108-15 cells expressed mRNA for the ecto-ATPase and nucleotide pyrophosphatase, but not for CD73. These results suggest that local adenosine formation by an ecto-enzyme distinct from CD73 is involved in adenine nucleotide-induced cyclic AMP formation in NG108-15 cells.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 84(3), 325-333, 2000-11-01

    The Japanese Pharmacological Society

References:  25

Cited by:  6

Codes

  • NII Article ID (NAID)
    10008186293
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    5586976
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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