Necessity of Enzymatic Activity of Alkaline Phosphatase for Mineralization of Osteoblastic Cells

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Author(s)

    • Sugawara Yuki SUGAWARA Yuki
    • Department of Oral Functional Science (Section of Orthodontics), Graduate School of Dental Medicine, Hokkaido University
    • Suzuki Kuniaki SUZUKI Kuniaki
    • Department of Oral Pathobiological Science (Section of Dental Pharmacology), Graduate School of Dental Medicine, Hokkaido University
    • ANDO Masaki
    • Department of Oral Functional Science (Section of Orthodontics), Graduate School of Dental Medicine, Hokkaido University
    • IIDA Junichiro
    • Department of Oral Functional Science (Section of Orthodontics), Graduate School of Dental Medicine, Hokkaido University

Abstract

Alkaline phosphatase (ALP) is supposed to be important for bone formation; however, its role is not clear. In this study, we examined the importance of enzymatic activity of ALP and anchoring of ALP protein to the cells for mineralization of an osteoblastic cell line, MC3T3-E1. While we cultured the cells in the presence of tetramisole, an inhibitor of ALP activity, ALP protein was expressed at a similar level to that in the control. Although tetramisole showed no effect on cell growth and increased hydroxyproline accumulation, it decreased the osteocalcin production and the accumulation of calcium and phosphate in the matrices. Tetramisole also inhibited mineralized nodule formation, which was observed by optical microscopy and detected by Von Kossa staining. On the other hand, when ALP protein was released from the cell membranes with the use of phosphatidylinositol-specific phospholipase C, no marked changes were detected in hydroxyproline, calcium and phosphate accumulations in the matrices at late calcification stage, which was consistent with the morphological findings. These results clearly show that enzymatic activity of ALP is necessary for mineralization of MC3T3-E1 cells, but not the presence of ALP protein or anchoring of ALP to the cells.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 88(3), 262-269, 2002-03-01

    The Japanese Pharmacological Society

References:  28

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008187710
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    6119450
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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