Competence Effect of Basic Fibroblast Growth Factor on Cell Cycle in Subcultured Endothelial Cells of Rat Aorta by Flow Cytometry

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Author(s)

    • KIMURA Ikuko
    • Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
    • OKABE Motonori
    • Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
    • OGASAWARA Masaru
    • Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
    • KIMURA Masayasu
    • Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

Abstract

With flow cytometry, we investigated the effect of basic fibroblast growth factor (bFGF)-induced competence in subcultured endothelial cells (EC) (4—9-passage) of rat thoracic aorta. The cell population in each phase of the cell cycle was determined by a double staining technique with fluorescein isothiocyanate-conjugated mouse monoclonal antibody against the proliferation-associated nucleus antigen Ki-67 and propidium iodide for total DNA content. EC were cultured in medium containing 5% fetal bovine serum (FBS) for 6 days. After serum-starvation for 2 days, the treatment with bFGF (3—10 ng/ml) for 12 hr promoted the entry of cells into the G<SUB>1</SUB> phase from the G<SUB>0</SUB> phase concentration-dependently. bFGF (10 ng/ml) increased the cell population in the G<SUB>1</SUB> phase by 50% of the total EC, compared with the control culture without bFGF. A further 12—15-hr culture with 1% FBS after bFGF treatment promoted the entry of the cell into the S phase. Thus flow cytometric analysis demonstrates that bFGF stimulates the entry of EC into the G<SUB>1</SUB> phase from the G<SUB>0</SUB> phase.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 71(1), 21-27, 1996-05

    The Japanese Pharmacological Society

References:  20

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008188200
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • Data Source
    CJP  CJPref  J-STAGE 
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