Prostanoid Receptor-Mediated Calcium Signaling in Cultured Rat Astrocytes
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We investigated prostanoid-induced intracellular Ca<SUP>2+</SUP> mobilization in Ca<SUP>2+</SUP>-sensitive dye fura-2-loaded cultured astrocytes. The thromboxane (TX) A<SUB>2</SUB> analog STA<SUB>2</SUB> (9, 11-epithio-11, 12-methanoTXA<SUB>2</SUB>) and/or prostaglandin (PG) F<SUB>2α</SUB> (each used at 1 μM) stimulated intracellular Ca<SUP>2+</SUP> mobilization in single cultured rat type 1 astrocytes. Three response patterns were observed: only STA<SUB>2</SUB>-sensitive, only PGF<SUB>2α</SUB>-sensitive, and both prostanoids-sensitive cells. The Ca<SUP>2+</SUP> response was prostanoid-dose-dependent (0.1-1 μM) and showed a rapid spike-like Ca<SUP>2+</SUP> rise that peaked within 30 sec after the stimulation by the ligand. These observations suggest that type 1 astrocytes are heterogeneous with respect to the expression of receptors for TXA<SUB>2</SUB> and PGF<SUB>2α</SUB>, which are linked to Ca<SUP>2+</SUP> mobilization.
- The Japanese Journal of Pharmacology
The Japanese Journal of Pharmacology 71(1), 85-87, 1996-05
The Japanese Pharmacological Society