Effects of ω-Conotoxin GVIA on the Activation of Capsaicin-Sensitive Afferent Sensory Nerves in Guinea Pig Airway Tissues

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Author(s)

    • MORIMOTO Hiroshi
    • Department of Pharmacology, New Drug Research Laboratories, Fujisawa Pharmaceutical Co., Ltd.
    • MATSUDA Atsushi
    • Department of Pharmacology, New Drug Research Laboratories, Fujisawa Pharmaceutical Co., Ltd.
    • OHORI Makoto
    • Department of Pharmacology, New Drug Research Laboratories, Fujisawa Pharmaceutical Co., Ltd.
    • FUJII Takashi
    • Department of Pharmacology, New Drug Research Laboratories, Fujisawa Pharmaceutical Co., Ltd.

Abstract

We examined the effects of Ca<SUP>2+</SUP> channel antagonists on various respiratory reactions induced by the activation of capsaicin-sensitive afferent sensory nerves. Intravenous (i.v.) injection of the N-type Ca<SUP>2+</SUP> channel antagonist ω-conotoxin GVIA (CgTX) (1-20 μg/kg) dose-dependently inhibited capsaicin-induced guinea pig bronchoconstriction, whereas i.v. administration of the L-type antagonist nicardipine (100 μg/kg), the P-type antagonist ω-agatoxin IVA (AgaTX) (20 μg/kg) or the OPQ family-type antagonist ω-conotoxin MVIIC (CmTX) (20 μg/kg) had no effect. However, CgTX (20 μg/kg) failed to inhibit substance P-induced guinea pig bronchoconstriction. CgTX (20 μg/kg) significantly inhibited cigarette smoke-induced guinea pig tracheal plasma extravasation, but not the substance P-induced reaction. CgTX also reduced electrical field stimulation-induced guinea pig bronchial smooth muscle contraction (0.01-10 μM) and capsaicin-induced substance P-like immunoreactivity release from guinea pig lung (0.14 μM). This evidence suggests that N-type Ca<SUP>2+</SUP> channels modulate tachykinin release from capsaicin-sensitive afferent sensory nerve endings in guinea pig airway tissue.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 71(2), 161-166, 1996-06

    The Japanese Pharmacological Society

References:  22

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008188655
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • Data Source
    CJP  CJPref  J-STAGE 
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