Affinity Purification of Macrophage Migration Inhibitory Factor/Glycosylation Inhibiting Factor (MIF/GIF) from Bovine Brain by Using a Peptide Ligand Derived from a Novel Serpin

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Author(s)

Abstract

We purified macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using an affinity column with the C-terminal region peptide of a novel serpin as a ligand. The affinity purified preparation showing a single band on SDS-PAGE contained four peptides on RP-HPLC, which were converged into two peptides time-dependently. Sequence analysis and Western blotting revealed that one was identical to bovine MIF/GIF and the other was an N-terminally modified form of MIF/GIF. These results indicated that there exist at least two forms of MIF/GIF in the bovine brain and that they have an affinity for the C-terminal portion of the serpin.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 71(3), 259-262, 1996-07

    The Japanese Pharmacological Society

References:  15

Codes

  • NII Article ID (NAID)
    10008189094
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    00215198
  • Data Source
    CJP  J-STAGE 
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