The Mode of Inhibitory Action of α-Mangostin, a Novel Inhibitor, on the Sarcoplasmic Reticulum Ca^<2+>-Pumping ATPase from Rabbit Skeletal Muscle

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Author(s)

    • FURUKAWA Ken-ichi
    • Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University
    • SHIBUSAWA Kazuki
    • Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University
    • CHAIRUNGSRILERD Nattaya
    • Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University
    • OHTA Tomihisa
    • Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Tohoku University
    • NOZOE Shigeo
    • Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Tohoku University
    • OHIZUMI Yasushi
    • Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University

Abstract

α-Mangostin, the principal ingredient of the fruit hull of <I>Garcinia mangostana</I>, caused a concentration-dependent decrease in the activities of both Ca<SUP>2+</SUP>-ATPase and Ca<SUP>2+</SUP>-transport of the sarcoplasmic reticulum from rabbit skeletal muscle with an IC<SUB>50</SUB> value of 5 μM. Neither Ca<SUP>2+</SUP> release nor other enzyme activities were affected by α-mangostin. Kinetic analysis of the inhibitory effects of α-mangostin on Ca<SUP>2+</SUP>-ATPase suggests that the inhibition of the ATPase is a noncompetitive-type with respect to ATP or Ca<SUP>2+</SUP>. α-Mangostin may become a useful pharmacological tool for clarifying the physiological functions of Ca<SUP>2+</SUP>-pumping ATPase and sarcoplasmic reticulum.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 71(4), 337-340, 1996-08-01

    The Japanese Pharmacological Society

References:  11

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008189358
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • Data Source
    CJP  CJPref  J-STAGE 
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