Expression of Low-Molecular-Weight Kininogen mRNA in Human Fibroblast WI38 Cells

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Author(s)

    • TAKANO Masaoki
    • Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University
    • KONDO Junya
    • Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University
    • YAYAMA Katsutoshi
    • Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University
    • OKAMOTO Hiroshi
    • Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe Gakuin University

Abstract

Expression of the low-molecular-weight kininogen (L-kininogen) mRNA in the human fibroblast cell line WI38 was examined by means of the reverse-transcription polymerase chain reaction and Southern blotting using human L-kininogen cDNA as a probe. The results demonstrated that WI38 fibroblasts expressed L-kininogen mRNA and that the expression was stimulated by 1 mM dibutyryl cAMP or 10 μM prostaglandin E<SUB>2</SUB>. Dexamethasone (1 μM) inhibited the stimulatory effect of prostaglandin E<SUB>2</SUB>. These results suggest that human fibroblasts supply L-kininogen, a protein precursor of the inflammatory mediator kinins, to connective tissues in response to inflammatory stimuli and that glucocorticoids may exert the antiinflammatory effect in part by inhibiting the local production of L-kininogen.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 71(4), 341-343, 1996-08-01

    The Japanese Pharmacological Society

References:  16

Codes

  • NII Article ID (NAID)
    10008189370
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    00215198
  • Data Source
    CJP  J-STAGE 
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