Pituitary Adenylate Cyclase-Activating Polypeptibe (PACAP) Receptor mRNA in the Rat Adrenal Gland: Localization by In Situ Hybridization and Identification of Splice Variants

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Author(s)

Abstract

The distribution of mRNA for pituitary adenylate cyclase-activating polypeptide receptor (PACAP-R) was examined by <I>in situ</I> hybridization in rat adrenal gland. In the adrenal medulla, PACAP-R mRNA was expressed in almost all chromaffin cells without any significant expression in the cortical region. Using the reverse transcription-polymerase chain reaction, we analyzed the mRNA expression of PACAP-R splice variant forms in the adrenal gland. The predominant forms observed were the variant having a 28amino acid insert in the third intracellular loop (termed PACAP-R-hopl). As PACAP is localized in the noradrenaline secreting cells of the adrenal chromaffin cells, and stimulates catecholamine release, the present results suggest that PACAP may serve as a paracrine or autocrine regulatory factor for the chromaffin cells through PACAP-R.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 75(2), 203-207, 1997-10-01

    The Japanese Pharmacological Society

References:  15

Cited by:  6

Codes

  • NII Article ID (NAID)
    10008189784
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4326421
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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