Effect of Human Neutrophil Elastase on Tracheal Mucociliary Transport in Anesthetized Quails

Access this Article

Author(s)

    • ISOHAMA Yoichiro
    • Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
    • TAKAHAMA Kazuo
    • Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University
    • MIYATA Takeshi
    • Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University

Abstract

We investigated the effect of human neutrophil elastase (HNE) on tracheal mucociliary transport in anesthetized quails. Topical application of HNE (30-300 μg/kg) to tracheal mucosa dosedependently decreased mucociliary transport velocity (MCTV). The HNE (300 μg/kg)-induced decrease in MCTV was blocked by ONO-5046·Na (sodium <I>N</I>-[2-[4-(2, 2-dimethylpropionyloxy)phenyl-sulfonylamino]benzoyl]aminoacetate tetrahydrate) (3-30 mg/kg, i.m.), a specific neutrophil elastase inhibitor. Furthermore, we found that HNE increased DNA, fucose and protein contents of tracheal lavages, and the increases were also reverted by ONO-5046·Na. These results indicated that HNE decreased tracheal mucociliary transport, and the decrease may be, at least in part, ascribed to the deterioration of tracheal secretions.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 75(4), 439-442, 1997-12-01

    The Japanese Pharmacological Society

References:  15

Codes

  • NII Article ID (NAID)
    10008190720
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    00215198
  • NDL Article ID
    4371111
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
Page Top