Effect of PPlD-1, a Synthetic Antiplatelet Compound, on Rabbit Platelets

• Liao Chang-Hui

  Pharmacological Institute, College of Medicine, National Taiwan University

• Ko Feng-Ning

  Pharmacological Institute, College of Medicine, National Taiwan University

• Kuo Sheng-Chu

  Graduate Institute of Pharmaceutical Chemistry, China Medical College

• Teng Che-Ming

  Pharmacological Institute, College of Medicine, National Taiwan University

書誌事項

タイトル別名

• Effect of PP1D-1, a Synthetic Antiplatelet Compound, on Rabbit Platelets.
• Effect of PP1D-1 a Synthetic Antiplatel

抄録

The antiplatelet mechanism of a synthetic compound, 2-chloro-3-methoxycarbonylpropionamido-1, 4-naphthoquinone (PP1D-1), was studied by employing washed rabbit platelets in vitro. PP1D-1 concentration-dependently inhibited thrombin (0.1 U/ml)-, platelet-activating factor (2 ng/ml)-, collagen (10 μg/ml)-, arachidonic acid (100 μM)- and U46619 (1 μM)-induced aggregation and ATP release in washed rabbit platelets. The IC₅₀ values of PP1D-1 for aggregation induced by the above inducers are 17.9±1.7, 9.8±1.1, 3.9±0.4, 1.8±0.3 and 1.7±0.3 μM, respectively. PP1D-1 did not affect platelet thromboxane B₂ or prostaglandin D₂ formation induced by arachidonic acid, indicating that it did not affect cyclooxygenase and thromboxane synthase activities. PP1D-1 significantly inhibited the formation of inositol 1, 4, 5-trisphosphate caused by these five platelet stimulators. Moreover, PP1D-1 inhibited the increase in intracellular calcium concentration induced by these agents. On the contrary, PP1D-1 did not inhibit thapsigargin-elevated intracellular calcium concentration in indomethacin-pretreated platelets, indicating it did not influence the effect of thapsigargin. According to these data, PP1D-1 exerts antiplatelet effects mainly by inhibiting phosphoinositide turnover.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 76 (2), 141-148, 1998

  公益社団法人 日本薬理学会

キーワード

• 2-Chloro-3-methoxycarbonylpropionamido-1, 4-naphthoquinone (PP1D-1)
• Platelet (rabbit)
• Inositol 1, 4, 5-trisphosphate (IP<SUB>3</SUB>)
• Intracellular calcium concentration
• Thapsigargin

詳細情報

• CRID: 1390282679262736128
• NII論文ID: 10008191395
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.76.141
• COI: 1:CAS:528:DyaK1cXht12ntrw%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 4416239
• PubMed: 9541276
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4416239
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819312223?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819312223?httpAccept=text/plain
• Web Site: https://www.jstage.jst.go.jp/article/jjp/76/2/76_2_141/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1998195131
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
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