Expression of Cytokine mRNA in Extrahepatic Organs in a Mouse Concanavalin A-Hepatitis Model

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Author(s)

Abstract

The liver injury in the concanavalin A (Con A)-induced mouse hepatitis model has been well studied. However, there has been little study on the effects of Con A on extrahepatic organs. The aim of the present work was to determine the effects of Con A on the spleen, kidney and lung. A histopathological study showed that Con A (15 mg/kg, i.v.) administration affects not only the liver, but also all these extrahepatic organs. Messenger RNA expression was studied by the using polymerase chain reaction. Treatment with Con A induced interleukin-2 mRNA in the spleen, but only slightly induced it in the kidney. The mRNAs of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were induced in all these organs. At 24 hr after Con A treatment, the expression of IFN-γ mRNA, but not that of TNF-α mRNA, was inhibited by cyclosporine A (50 mg/kg, i.p.), suggesting that Con A induced these cytokine mRNAs through different mechanisms. In the kidney and lung, CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T-cell infiltration was suggested by the Con A-induced CD4 and CD8 mRNAs. The present study showed the histopathological effects of Con A and Con A-induced cytokine mRNA expression on the spleen, kidney and lung.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 77(3), 219-225, 1998-07-01

    The Japanese Pharmacological Society

References:  16

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008193235
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4525738
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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