Effect of Gadolinium Chloride Treatment on Concanavalin A-Induced Cytokine mRNA Expression in Mouse Liver
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The effect of Kupffer cell depression on concanavalin A (Con A)-induced cytokine mRNA expression in the liver was studied. Gadolinium chloride (GdCl<SUB>3</SUB>) is a commonly used Kupffer cell inhibitor. GdCl<SUB>3</SUB> (40 mg/kg, i.p.) was injected into each mouse, and 24 hr later, Con A (0.2 mg/mouse) was administered. Plasma was obtained at 24 hr after Con A treatment for alanine aminotransferase (ALT) measurement. GdCl<SUB>3</SUB> treatment inhibited Con A-induced elevation of ALT. However, it did not inhibit Con A-induced interleukin-2 or tumor necrosis factor-α mRNA expression. The present results suggest that Kupffer cells are not responsible for Con A-induced cytokine expression in the liver.
- The Japanese Journal of Pharmacology
The Japanese Journal of Pharmacology 78(1), 101-103, 1998-09
The Japanese Pharmacological Society