Hydrogen Peroxide-Induced Apoptosis and Necrosis in Human Lung Fibroblasts : Protective Roles of Glutathione

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Author(s)

Abstract

Although reactive oxygen species (ROS)-related cell damage has been implicated in pathogenesis of fibrogenetic pulmonary disorders, features of ROS-mediated cell death in human lung fibroblasts are not completely understood. We therefore examined the effects of hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) on cell growth kinetics in human lung fibroblasts (HFL-1 cells) and tested the roles of antioxidants on the H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death (i.e., necrosis and apoptosis) in HFL-1 cells. We found that the relatively low concentrations of H<SUB>2</SUB>O<SUB>2</SUB> ranging from 10 μM to 100 μM induced predominantly apoptosis, whereas higher concentration of H<SUB>2</SUB>O<SUB>2</SUB> ranging 1 mM - 10 mM induced predominantly necrosis in HFL-1 cells. Extracellular supplementation of glutathione (GSH) in culture media significantly abolished the H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death, whereas GSH-depleted cells by pretreatment with buthionine sulfoxime (BSO) were likely to undergo cell death caused by a lower concentration of H<SUB>2</SUB>O<SUB>2</SUB> than normal HFL-1 cells without BSO treatment. These results indicate that H<SUB>2</SUB>O<SUB>2</SUB> induces both necrosis and apoptosis of human lung fibroblasts at least in part through the action of ROS and that modulation of the ROS production inside and outside of cells may influence the cell survival during oxidative insults.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 79(1), 33-40, 1999-01

    The Japanese Pharmacological Society

References:  31

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008194067
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4636892
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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