Inhibitory Effect of Vitamin K_2 (Menatetrenone) on Bone Resorption in Ovariectomized Rats : A Histomorphometric and Dual Energy X-Ray Absorptiometric Study

Access this Article



To clarify how vitamin K<SUB>2</SUB> prevents bone loss in vivo, it was given to ovariectomized 20-week-old rats for 2 weeks. Bone mineral density (BMD) in the whole femur and in 7 specific portions (F1 to F7 from the proximal to the distal end) was determined by dual-energy X-ray absorptiometry, and histomorphometry was also performed in proximal tibial metaphysis. Ovariectomy (OVX) resulted in significant decreases in the BMD in the whole femur and the F1, F2, F6 and F7 portions. Histomorphometrical analysis of the tibia showed that the bone volume / tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N) were decreased, while trabecular separation (Tb.Sp) and osteoclast number / bone surface (Oc.N/BS) were increased by OVX. The parameters for bone formation were not changed by OVX. These data indicate that the bone loss within 2 weeks is due to the enhancement of bone resorption. Vitamin K<SUB>2</SUB> at 50 mg/kg inhibited the decrease in the BMD of the whole femur together with the F6 and F7 portions. Vitamin K<SUB>2</SUB> also inhibited the decrease in Tb.N and the increases in Tb.Sp, Oc.N/BS and osteoclast surface / bone surface (Oc.S/BS) caused by OVX. These results suggest that vitamin K<SUB>2</SUB> prevents bone loss through the inhibition of bone resorption and osteoclast formation in vivo.


  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 80(1), 67-74, 1999-05-01

    The Japanese Pharmacological Society

References:  25

Cited by:  9


  • NII Article ID (NAID)
  • Text Lang
  • Article Type
    Journal Article
  • ISSN
  • NDL Article ID
  • NDL Source Classification
  • NDL Call No.
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top