Pharmacological Comparison Between the Nitrergic Responses Produced by Intramural Nerve Stimulation and Exogenous NO-Donors in Rat Gastric Fundus

Access this Article

Author(s)

Abstract

To investigate whether the nitrergic nerve-mediated smooth muscle relaxation is caused by authentic nitric oxide (NO) and is mediated via guanosine 3':5'-cyclic monophosphate (cyclic GMP), we compared the response to electrical field stimulation of nitrergic nerve (EFS) with other NO-related responses in rat gastric fundus strips. EFS, sodium nitroprusside (SNP), <I>S</I>-nitroso-<I>N</I>-acetylpenicillamine (SNAP), and acidified NaNO<SUB>2</SUB> and inducible NO synthase (iNOS)-mediated NO all produced relaxation and elevated cyclic GMP level in rat fundus strips. However, the basal and stimulated cyclic GMP levels were significantly lower than the basal level in aorta (40±4 pmol/g wet tissue). Methylene blue and 6-anilino-5, 8-quinolinedione (LY83583), both known as soluble guanylyl cyclase inhibitors and O<SUB>2</SUB><SUP>-</SUP> generators that scavenge NO, reduced the elevation of cyclic GMP level by all stimuli and inhibited the relaxations only in response to NaNO<SUB>2</SUB> and iNOS-mediated NO but not to the other stimuli. These results suggest that in the rat gastric fundus strips the relaxations induced by not only nitrergic nerve but also SNP and SNAP are not associated with cyclic GMP production, in contrast to the relaxations mediated by authentic NO.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 80(2), 155-161, 1999-06-01

    The Japanese Pharmacological Society

References:  34

Codes

  • NII Article ID (NAID)
    10008194964
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    4771970
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
Page Top