Ameliorative Effect of Adenosine on Hypoxia-Reoxygenation Injury in LLC-PK_1, a Porcine Kidney Cell Line

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Author(s)

Abstract

We studied the effects of adenosine on injury caused by hypoxia and reoxygenation in LLC-PK<SUB>1</SUB> cells. Lactate dehydrogenase and γ-glutamyltranspeptidase were released from cells exposed to hypoxia for 6 hr and then reoxygenation for 1 hr. The addition of adenosine at 100 μM to the medium before hypoxia began significantly decreased enzyme leakage into medium during both hypoxia and reoxygenation. The adenosine A<SUB>1</SUB>-receptor agonist, <I>R</I>(-)-<I>N</I><SUP>6</SUP>-(2-phenylisopropyl)adenosine (R-PIA), at the concentration of 100 μM, did not affect enzyme release, but the adenosine A<SUB>2</SUB>-receptor agonist 2-<I>p</I>-[2-carboxyethyl]phenethyl-amino-5'-<I>N</I>-ethylcarboxamido-adenosine hydrochloride (CGS 21680) at the concentration of 100 nM, suppressed the injury caused by hypoxia and reoxygenation. There were decreases in cAMP contents and ATP levels in LLC-PK<SUB>1</SUB> cells injured by hypoxia and reoxygenation. Adenosine (100 μM) restored ATP levels in the cells during reoxygenation. With adenosine, the intracellular cAMP level was increased prominently during reoxygenation. These results suggest that adenosine protects LLC-PK<SUB>1</sub> cells from injury caused by hypoxia and reoxygenation by increasing the intracellular cAMP level via adenosine A<SUB>2</SUB> receptor.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 80(2), 163-167, 1999-06-01

    The Japanese Pharmacological Society

References:  27

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008194999
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4771981
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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