Protective Effect of Histidine on Hydroxyl Radical Generation Induced by Potassium-Depolarization in Rat Myocardium

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Author(s)

Abstract

We investigated the efficacy of histidine on potassium-depolarization induced hydroxyl radical (·OH) generation in the extracellular fluid of rat myocardium by a flexibly mounted microdialysis technique (O system). After the rat was anesthetized, a microdialysis probe was implanted in the left ventricular myocardium, and then sodium salicylate in Ringer’s solution (0.5 nmol/μl per minute) was infused to detect the generation of ·OH as reflected by the nonenzymatic formation of 2, 3-dihydroxybenzoic acid (DHBA). Infusion of KCl (70 mM) clearly produced an increase in ·OH formation. However, when KCl in the presence of a high concentration of histidine (25 mM) was infused through the microdialysis probe, KCl failed to increase the 2, 3-DHBA formation. To examine the effect of histidine on ischemia-reperfusion of the myocardium, the heart was subjected to myocardial ischemia for 15 min by occlusion of the left anterior descending coronary artery (LAD). When the heart was reperfused, a marked elevation of the levels of 2, 3-DHBA was observed in the heart dialysate. However, when corresponding experiments were performed with histidine (25 mM)-pretreated animals, histidine prevented the ischemia-reperfusion induced ·OH formation trapped as 2, 3-DHBA. These results indicate that histidine may protect against K<SUP>+</SUP>-depolarization-evoked ·OH generation in rat myocardium.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 80(3), 217-222, 1999-07

    The Japanese Pharmacological Society

References:  36

Codes

  • NII Article ID (NAID)
    10008195231
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    4806269
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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