Role of Glutamate Receptors and Voltage-Dependent Calcium Channels in Glutamate Toxicity in Energy-Compromised Cortical Neurons.
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- Kimura Manami
- Eisai Tsukuba Research Laboratories
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- Katayama Kouichi
- Eisai Tsukuba Research Laboratories
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- Nishizawa Yukio
- Eisai Tsukuba Research Laboratories
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Abstract
We have examined the effect of glutamate receptor antagonists and voltage-dependent calcium channel blockers on the neuronal injury induced by the combination of a low concentration of N-methyl-D-aspartate (NMDA) or kainate and energy compromise resulting from the use of glucose-free incubation buffer. Toxicity induced by NMDA or kainate was enhanced in the glucose-free buffer. NMDAor non-NMDA-receptor antagonists added to the glucose-free buffer at the same time inhibited the neuronal cell death induced by each agonist. An NMDA-receptor antagonist, MK-801, but not non-NMDA-receptor antagonists, inhibited the toxicity when added to the culture medium after exposure of the cells to the agonists. P/Q-type calcium channel blockers, ω-agatoxin IVA and ω-agatoxin TK, and an N-type calcium channel blocker, ω-conotoxin GVIA, significantly attenuated the neuronal injury, although an L-type calcium channel blocker, nifedipine, showed little neuroprotective effect. A combination of calcium channel blockers of the three subtypes showed the most prominent neuroprotective effect. These observations suggest that the overactivation of NMDA and non-NMDA receptors and consequent activation of the voltage-dependent calcium channels lead to neuronal cell death in energy-compromised cortical neurons.
Journal
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- The Japanese Journal of Pharmacology
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The Japanese Journal of Pharmacology 80 (4), 351-358, 1999
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390282679261589632
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- NII Article ID
- 10008195828
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- NII Book ID
- AA00691188
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- COI
- 1:CAS:528:DyaK1MXlslWlt7g%3D
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- ISSN
- 13473506
- 00215198
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- NDL BIB ID
- 4837021
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- PubMed
- 10496336
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I4837021
- https://api.elsevier.com/content/article/PII:S0021519819308315?httpAccept=text/xml
- https://api.elsevier.com/content/article/PII:S0021519819308315?httpAccept=text/plain
- https://www.jstage.jst.go.jp/article/jjp/80/4/80_4_351/_pdf
- https://search.jamas.or.jp/link/ui/2000023876
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed