Vasodilating Effect and Tissue Accumulation of Prostaglandin E_1 Incorporated in Lipid Microspheres on the Rat Ductus Arteriosus

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Author(s)

Abstract

Prostaglandin E<SUB>1</SUB> incorporated in lipid microspheres (lipo PGE<SUB>1</SUB>) was administered to the umbilical vein of neonatal rats. Morphological measurement and quantitative autoradioluminography assessed the relationship between the vasodilating effect and tissue accumulation of lipo PGE<SUB>1</SUB> in the ductus arteriosus. In the morphological measurement under microscopy, the inner diameter ratio of the ductus arteriosus to the main pulmonary artery after infusion of <SUP>3</SUP>H-labeled lipo PGE<SUB>1</SUB> (<SUP>3</SUP>H-lipo PGE<SUB>1</SUB>) continued to remain significantly higher than that of free <SUP>3</SUP>H-PGE<SUB>1</SUB>. Autoradioluminography of the frozen frontal section of neonates after intravenous infusion of <SUP>3</SUP>H-lipo PGE<SUB>1</SUB> for 2 h revealed that the ductus levels of radioactivity were higher than those of free <SUP>3</SUP>H-PGE<SUB>1</SUB> in saline solution, although the blood levels were almost equal. Localization of lipo PGE<SUB>1</SUB> labeled with a lipophilic fluorescent probe, 1, 1′-dioctadecyl-3, 3, 3′, 3′-tetramethyl-indocarbocyanine perchlorate (diI), in the endothelial cells of the ductus arteriosus was confirmed by confocal laser scanning microscopy. These findings suggest that the incorporation of lipid microspheres by the endothelial cells is one of the mechanisms that enables lipo PGE<SUB>1</SUB> to accumulate to higher levels in the ductus tissue and to act more efficiently than free PGE<SUB>1</SUB> in neonatal rats.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 81(1), 107-114, 1999-09

    The Japanese Pharmacological Society

References:  21

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008196364
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4867923
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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