Maitotoxin-Induced Phosphoinositide Hydrolysis Is Dependent on Extracellular but Not Intracellular Ca^<2+> in Human Astrocytoma Cells  [in Japanese]

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Author(s)

Abstract

Since maitotoxin, a potent marine toxin, is known to cause not only Ca<SUP>2+</SUP> influx but also phosphoinositide hydrolysis, we investigated the Ca<SUP>2+</SUP> dependency of maitotoxin-induced phosphoinositide hydrolysis in 1321N1 human astrocytoma cells. Maitotoxin elicited inositol 1, 4, 5-trisphosphate accumulation in a time-dependent manner. In [<SUP>3</SUP>H]inositol-labeled cells, maitotoxin stimulated phosphoinositide hydrolysis in an extracellular Ca<SUP>2+</SUP>-dependent manner. Maitotoxin also caused an intracellular Ca<SUP>2+</SUP> elevation, which was abolished by an intracellular Ca<SUP>2+</SUP> chelater BAPTA-AM. Interestingly, maitotoxin still caused phosphoinositide hydrolysis in the BAPTA-AM-treated cells. These results indicate that maitotoxin-induced phosphoinositide hydrolysis is dependent on extracellular but not intracellular Ca<SUP>2+</SUP> in 1321N1 human astrocytoma cells.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 81(2), 240-243, 1999-10

    The Japanese Pharmacological Society

References:  15

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008197056
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4886740
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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