Comparison of the Effects of Endothelin-1, -2 and -3 (1-31) on Changes in [Ca^<2+>]_i in Human Coronary Artery Smooth Muscle Cells

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We have previously found that human chymase selectively cleaves big endothelins (ETs) at the Tyr<SUP>31</SUP>-Gly<SUP>32</SUP> bond to produce 31-amino-acid endothelins, ETs (1 - 31). In the present study, we investigated the effects of ETs (1 - 31) on changes in intracellular free Ca<SUP>2+</SUP> ([Ca<SUP>2+</SUP>]<SUB>i</SUB>) in cultured human coronary artery smooth muscle cells (HCASMCs) using confocal laser microscopy. ETs (1 - 31) increased [Ca<SUP>2+</SUP>]<SUB>i</SUB> in a concentration-dependent manner. Phosphoramidon did not inhibit the increases in [Ca<SUP>2+</SUP>]<SUB>i</SUB> caused by ETs (1 - 31). The [Ca<SUP>2+</SUP>]<SUB>i</SUB> increases induced by ETs (1 - 31) were compared to those of ETs (1 - 21) and big ETs. ET-1 (1 - 21) was about 10-times more potent than big ET-1 or ET-1 (1 - 31), whereas big ET-2 was 10-times less potent than ET-2 (1 - 21) or ET-2 (1 - 31). ETs (1 - 31) may induce [Ca<SUP>2+</SUP>]<SUB>i</SUB> increase through ET<SUB>A</SUB>-type or ET<SUB>A</SUB>-type-like receptors. The 10<SUP>-12</SUP> M ET (1 - 31)-induced increases in [Ca<SUP>2+</SUP>]<SUB>i</SUB> were not affected by removal of extracellular Ca<SUP>2+</SUP>, but were inhibited by thapsigargin. These results suggested that ET-1, -2 and -3 (1 - 31) showed similar potencies in increasing [Ca<SUP>2+</SUP>]<SUB>i</SUB> and mechanisms of ET (1 - 31)-induced increases in [Ca<SUP>2+</SUP>]<SUB>i</SUB> may be similar among the three ETs (1 - 31).

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 81(3), 298-304, 1999-11

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008197322
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00215198
  • NDL 記事登録ID
    4918367
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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