Influence of Chronic Treatment With Imipramine on mRNA Levels in Rat Brain : Elevation of Glyceraldehyde-3-phosphate Dehydrogenase Levels

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Author(s)

Abstract

The differential display method was used to identify the intrinsic factor that changes its mRNA expression level in rat brain after a 14-day oral administration of 20 mg/kg imipramine. The expression of a 180-bp band was markedly enhanced by imipramine. The results of sequencing and a data base search revealed that the isolated clone was glyceraldehyde-3-phosphate dehydrogenase (GAPDH) with a one-base difference. Enhancement of the expression by imipramine was observed in the amygdala. Quantitative PCR showed that imipramine treatment significantly elevated the GAPDH/β-actin ratio in the cortex. These findings suggest that long-term treatment with imipramine stimulates GAPDH mRNA expression.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 81(4), 393-396, 1999-12-01

    The Japanese Pharmacological Society

References:  15

Codes

  • NII Article ID (NAID)
    10008197776
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    00215198
  • NDL Article ID
    4948614
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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