クコ果実のアラビノガラクタン-プロテインについて Isolation and Characterization of Arabinogalactan-protein from the Fruit of Lycium chinense Mill

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Abstract

クコの実からの冷水抽出物をDEAE-cellulose column chromatographyに供したところ,2種類のarabinogalactan-protein(AGP)が得られた.一つはarabinose:galactose=3:1からなり,分子量は42,000で,糖は蛋白質のserineおよびthreonineの2種のアミノ酸残基とグリコシド結合している糖蛋白質であり,もう一つはarabinose:galactose=1:1からなり,分子量は23,000で,糖は蛋白質のserine残基にグリコシド結合している糖蛋白質であることが明らかになった.さらに構造解析の結果,両者はともに分岐したtypeIIに属するarabinogalactanであるが,大きな相違点は(1→6)結合が主に分岐として存在(Cp-1-C)するか直鎖として存在(Cp-1-D)するかの違いであることが明らかとなった.

Two kinds of arabinogalactan-protein (Cp-1-C and -D) were obtained from the fruit of Lycium chinense Mill. In Cp-1-C, the carbohydrate was composed of arabinose and galactose at a ratio of 3:1, and O -glycosidically linked to both the serine and threonine residues of protein. Its molecular weight was 42, 000. In Cp-1-D, the ratio of arabinose to galactose was 1:1, and the O -glycosidical junction between the carbohydrate and protein was composed of serine residue. The molecular weight was 23, 000. From the results of structural characterization, both Cp-1-C and -D were assigned to type II arabinogalactan-protein (AGP), where a (1→6) linkage of galactose occurred mainly at the branching point in the case of Cp-1-C and mainly at the linear part in Cp-1-D.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 47(2), 155-161, 2000-06-30

    The Japanese Society of Applied Glycoscience

References:  21

Codes

  • NII Article ID (NAID)
    10008251700
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13403494
  • NDL Article ID
    5436001
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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