イソアミラーゼによるウルチ澱粉の部分加水分解物よりアミロペクチン外部層に存在する鎖長構造の直接分析 Direct Analysis of Chains on Outer Layer of Amylopectin through Partial Hydrolysis of Normal Starch by Isoamylase

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Abstract

アミロペクチンの構造情報を直接取得する簡便な分析方法を確立する目的で,イソアミラーゼによるウルチトウモロコシ澱粉から切り出される短鎖アミロース鎖を調べた.アミロースとアミロペクチンを分離せずに得られる部分加水分解物を,Toyopearl HW-50Sのゲルクロマトグラフィーにより3区分(fr.1,fr.2,fr.3)に分けた.短い鎖長を含むfr.3の鎖長分布をHPAEC-PADで分析した.fr.3に含まれる短鎖アミロースはアミロペクチンのほかに分岐アミロース由来のものも含まれるが,後者由来のものはわずかであると推定した.このアミロペクチンの一般的な構造情報を直接得る分析法は,日本で栽培された代表的な米澱粉で応用性を調べた.

Short-amylose chains released from normal corn starch with isoamylase were studied to establish an analytical method for getting directly structural information of amylopectin. The partial hydrolysates obtained without separation of amylose and amylopectin were fractionated into three fractions (fr.1, fr. 2 and fr. 3) by gel chromatography of Toyopearl HW-50S. The chain-length distribution of the fr. 3 containing shortish chains was analyzed by using HPAEC-system equipped with a pulsed amperometric detector (HPAEC-PAD). Although the shortamylose chains in the fr. 3 came from not only amylopectin but also branched amylose, it was estimated that the chains relating to branched amylose were minor in the hydrolysate. The analytical method getting directly a general information of amylopectin was conducted to typical rice starches produced in Japan as an applied experiment.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 48(2), 123-130, 2001-04-01

    The Japanese Society of Applied Glycoscience

References:  13

Codes

  • NII Article ID (NAID)
    10008252520
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13403494
  • NDL Article ID
    5764310
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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