Thermoanaerobacter brockii由来トレハロースホスホリラーゼによるα-D-ガラクトシルα-D-グルコシドの効率的酵素合成 Efficient Enzymatic Synthesis of Disaccharide, α-D-Galactosyl α-D-Glucoside, by Trehalose Phosphorylase from Thermoanaerobacter brockii

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Abstract

トレハロースをグルコシル供与体,ガラクトースを受容体として,好熱嫌気性菌Thermoanaerobacter brockii由来トレハロースホスホリラーゼを用いて非還元性二糖を酵素的に合成した.本糖質をパン酵母処理によるグルコースの除去,トレハラーゼ消化,アルカリ処理,分取HPLCを用いて効率良く反応液から単離した.酸分解,メチル化分析,<SUP>13</SUP>C-NMRなどにより本二糖の構造をα-D-ガラクトピラノシルα-D-グルコピラノシドと決定した.本糖質はα-ガラクトシダーゼによりごくわずか加水分解されたが,α-またはβ-グルコシダーゼ,β-ガラクトシダーゼ,トレハラーゼでは分解をうけなかった.

A non-reducing disaccharide was synthesized by trehalose phosphorylase from a thermophilic anaerobe, Thermoanaerobacter brockii, using trehalose as a glucosyl donor and D-galactose as an acceptor.This disaccharide was efficiently isolated from the reaction mixture by glucose eliminationusing baker's yeast, trehalase digestion, alkaline treatment and preparative HPLC. The chemicalstructure of the disaccharide was determined to be α-D-galactopyranosyl α-D-glucopyranoside byacid hydrolysis, methylation analysis, and by <SUP>13</SUP>C-NMR spectrometry. This saccharide was onlyminimally hydrolyzed by α-galactosidase, but not hydrolyzed by α- or β-glucosidase, β-galactosidase and trehalase.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 48(2), 135-137, 2001-04-01

    The Japanese Society of Applied Glycoscience

References:  10

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008252541
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13403494
  • NDL Article ID
    5764418
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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