α-アミラーゼの耐酸性タイプと中性タイプについて : クエン酸麹耐酸性α-アミラーゼの特性  [in Japanese] Study on Two Types of α-Amylase, the Acid-stable and the Neutral : Characterization of Acid-stable α-Amylase from Citric Acid Koji  [in Japanese]

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Author(s)

    • 菅沼 俊彦 SUGANUMA Toshihiko
    • 鹿児島大学農学部生物資源化学科 Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

Abstract

In Kagoshima, citric acid and shochu are produced on a commercial basis by the koji of Aspergillus groups. The black Aspergillus groups are known to produce two types of a-amylase, the acid-stable and the neutral, besides glucoamylase. In this study, we purified the acid-stable enzyme (UAA) from the acidic extract of the citric acid koji, and studied some enzymatic properties, especially the action pattern towards maltooligosaccharides, by comparison with the neutral enzyme (UNA) from the koji. Product analysis showed that UAA demonstrated a distinct cleavage pattern towards oligosaccharides more than G4, which suggests that UAA has a different subsite structure from the ordinary neutral a-amylase such as Taka-amylase A. The number of subsite is estimated to be 5, and subsite affinities of Al, A2, and A5 were evaluated to be 1.2 kcal/mol, 1.4 kcal/mol and 2.1 kcal/mol. Experiments with chemical modification suggested that UAA has a residue of Leu instead of Lys209 on Taka-amylase A, and a residue of Glu instead of His2l0. A new substrate Gal-G3-CNP was enzymatically synthesized from lactose and G3-CNP in order to discriminate between the two a -amylases, the acid-stable and the neutral.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 48(2), 187-193, 2001-04-01

    The Japanese Society of Applied Glycoscience

References:  9

Codes

  • NII Article ID (NAID)
    10008252675
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    13403494
  • NDL Article ID
    5764883
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  NDL  J-STAGE 
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