好熱性放線菌Thermoactinomyces vulgaris R-47のデキストリン利用に関与する遺伝子クラスターのクローニングとサイクロデキストリン結合タンパク質の性質

• ヨピ

  東京農工大学農学部応用生物科学科

• 殿塚 隆史

  東京農工大学農学部応用生物科学科

• 酒井 坦

  静岡県立大学大学院生活健康科学研究科

• 坂野 好幸

  東京農工大学農学部応用生物科学科

書誌事項

タイトル別名

• Cloning of a Gene Cluster for Dextrin Utilization from Thermoactinomyces vulgaris R-47 and Characterization of the Cyclodextrin-binding Protein.

抄録

To investigate the physiological role of an α-amylase, TVA II from Thermoactinomyces vulgaris R-47, which hydrolyze cyclodextrins and pullulan, a region located upstream of the TVA II gene was cloned and sequenced. Five open reading frames, designated as ORF-1 to -5, were found in a fragment of about 5 kbp. Three of these genes, ORF-1, -2 and -3, were homologous to the genes which encode proteins related to the sugar metabolic systems, such a maltose system of Esch erichia coli and cyclodextrin metabolic system of Klebsiella oxytoca. An expression vector for the ORF-3 protein, which was similar to maltose-binding protein from E. coli (MalE) and a cyclodextrinbinding protein from K. oxytoca (CymE), was constructed, and the expressed ORF-3 protein was purified. The ORF-3 protein was a cyclodextrin-binding protein because of having a higher binding ability for cyclodextrins than for maltose.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 49 (2), 107-114, 2002

  日本応用糖質科学会

詳細情報

• CRID: 1390001206293527040
• NII論文ID: 10008253471
• NII書誌ID: AN10453916
• ISSN: 13403494
• ISSN: 18807291
• ISSN: 13447882
• ISSN: http://id.crossref.org/issn/13447882
• DOI: 10.5458/jag.49.107
• COI: 1:CAS:528:DC%2BD38Xkt1Cisrk%3D
• NDL書誌ID: 6142247
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010650547
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6142247
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/49/2/49_2_107/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可