Regulation of Enzyme Activities by Free Mg〔2+〕 Concentration--Regulation of Stromal Fructose-1,6-bisphosphatase and Ribulose 1,5-Bisphosphate Carboxylase Activities

• Ishijima Sumio

  College of Agriculture and Graduate School of Agriculture, The Prefecture University of Kyoto

• Ohnishi Masatake

  College of Agriculture and Graduate School of Agriculture, The Prefecture University of Kyoto

書誌事項

タイトル別名

• Regulation of Enzyme Activities by Free Mg2+ Concentration. Regulation of Stromal Fructose-1,6-bisphosphatase and Ribulose 1,5-Bisphosphate Carboxylase Activities.
• —Regulation of Stromal Fructose-1, 6-bisphosphatase and Ribulose 1, 5-Bisphosphate Carboxylase Activities—

抄録

Although Mg is abundant within the cell, most is chelated to various organic molecules; only a small fraction of intracellular Mg is in the free (ionized) form, Mgtt Free Mg²⁺ regulates manyenzyme activities in cells. The effect of free Mg²⁺ concentration on the activities of spinach hloroplast fructose-1, 6-bisphosphatase (FBPase) and ribulose 1, 5-bisphosphate carboxylase (rubisco) was examined. Free Mg²⁺ concentrations in the assay mixtures were directly measured by a Mgtsensitive dye, mag-fura-2. FBPase was activated by a physiological concentration range of free Mgt, but the activation of rubisco was not observed. These results suggest that in illuminated chloroplasts, the increase in free Mg²⁺ activates FBPase, and this may be a physiological factor to stimulate CO₂ fixation.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 49 (2), 199-203, 2002

  日本応用糖質科学会

詳細情報

• CRID: 1390282681269968512
• NII論文ID: 10008253713
• NII書誌ID: AN10453916
• DOI: 10.5458/jag.49.199
• ISSN: 18807291
• ISSN: 13447882
• NDL書誌ID: 6142482
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6142482
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/49/2/49_2_199/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可