Characterization of Trypsin Modified with Dextran-dialdehyde

• TAKATSU Koichiro

• KOBAYASHI Mikihiko

  National Food Research Institute

Bibliographic Information

Other Title

• デキストランージアルデヒド修飾トリプシンの特性
• Characterization of Trypsin Modified wi

Abstract

Periodate oxidation of clinical dextran gave dextran-dialdehyde, which had absorption at the 1740cm-1 region of the infrared spectrum and contained 62% of D-glucose residues in oxidized form. Bovine pancreatic trypsin was modified with dextran-dialdehyde. The molecular weight of the modified trypsin was estimated to be 92, 000 based on the protein standards. The isoelectric point (p1) of trypsin decreased from 10.1 to 6.7 upon modification. Ten residues out of 15 free amino groups were modified per trypsin molecule. Carbohydrate content of modified trypsin was 49%. The modified trypsin showed a similar hydrolysis pattern of insulin B-chain with that of the native trypsin.

Journal

• Journal of Applied Glycoscience

  Journal of Applied Glycoscience 43 (1), 43-49, 1996

  The Japanese Society of Applied Glycoscience

Details

• CRID: 1390001205172018176
• NII Article ID: 10008254912
• NII Book ID: AN10453916
• DOI: 10.11541/jag1994.43.43
• ISSN: 18844898
• ISSN: 13403494
• NDL BIB ID: 3959776
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3959776
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed