高濃度のグルコースオキシダーゼにとよるウロン酸の酸化

• 小林 幹彦

  農林水産省食品総合研究所

• 西原 宏史

  茨城大学農学部

• 小林 昭一

  農林水産省食品総合研究所

書誌事項

タイトル別名

• Oxidation of Uronic Acids by a Large Excess of Glucose Oxidase Preparations.

抄録

Three commercial enzyme preparations of glucose oxidase (GOD) showed an oxidizing ability of glucuronic acid and galacturonic acid to form sugar acids. The optimum conditions of GOD reaction were at pH 8.0 and 50°C, whereass those of uronic acid oxidase (UOD) reaction were at pH 3.5 and 40°C. In spite of extensive attempts to separate GOD from UOD, the isolation of each activity was unsuccessful, and UOD reaction resulted from the wide substrate specificity of GOD reaction. More than 6-fold higher K_m values for glucuronic acid and galacturonic acid than for glucose might suggest that UOD reaction was the alternative reaction of GOD enzyme. However, a more definite conclusion on UOD activity should be drawn from further studies. The reaction products from uronic acids were analyzed by HPLC and paper chromatography, and some products were shown to be identical with sugar acids.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 46 (1), 1-7, 1999

  日本応用糖質科学会

詳細情報

• CRID: 1390282681270090880
• NII論文ID: 10008258526
• NII書誌ID: AN10453916
• ISSN: 13403494
• ISSN: 18807291
• ISSN: 13447882
• DOI: 10.5458/jag.46.1
• NDL書誌ID: 4785602
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030590194
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4785602
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/46/1/46_1_1/_pdf
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可