Thermoanaerobium brockiiの無細胞系によるトレハロースからの非還元性三糖,セラギノースの生成 Formation of a Nonreducing Trisaccharide, Selaginose, from Trehalose by a Cell-free System of Thermoanaerobium brockii

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Abstract

嫌気性好熱菌Thermoanaerobium brockii ATCC35047の菌体抽出液をトレハロースに作用させたところ,非還元性三糖の生成が認められた.本糖質を単離・精製し,部分酸加水分解,メチル化分析,<SUP>13</SUP>C-NMR分析により構造解析した結果,2-O-α-D-glucopyranosyl-α-D-glucopyranosyl α一D-glucopyranoside (セラギノース)であることが分かった.菌体抽出液からDEAE-Toyopearlカラムクロマトグラフィーにより,セラギノースの生成に関与する2種類の酵素の部分精製標品を得た.一つは既知酵素,トレハロースホスホリラーゼで,もう一つは,β 一グルコース1リン酸(β-GIP)とグルコースとからコージビオースを生成する活性を有する新規ホスホリラーゼであると考えられた.セラギノースはこれら2種類のホスホリラーゼの共同的作用によりトレハロースからβ一GIPを介して生成すると推定された.

A nonreducing trisaccharide was formed in a reaction mixture containing trehalose and the cell-free extract of a thermophilic anaerobe, Thermoanaerobium brockii ATCC 35047. This saccharide was isolated from the mixture by trehalase digestion, alkaline treatment and preparative HPLC. From the results of partial acid hydrolysis, methylation analysis, and <SUP>13</SUP>C-NMR analysis, the chemical structure of the saccharide was determined to be 2-O-α-D-glucopyranosyl α-D-glucopyranosyl α-D-glucopyrano- side (selaginose). Two enzymes related to Selaginose synthesis were partially purified by DEAEToyopearl column chromatography from the cell-free extract of T brockii. One was a trehalose phosphorylase (EC 2.4.1.64), which catalyzes the reversible phosphorolysis of trehalose into β-glucose-1-phosphate and glucose. The other synthesized kojibiose from β-glucose 1-phosphate and glucose. The latter enzyme was considered to be a novel phosphorylase. The hypothetical mechanism of selaginose synthesis was proposed as follows: trehalose+Pi→glucose+β-glucose 1-phosphate, β-glucose 1-phosphate +trehalose→selaginose+Pi.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 46(2), 129-134, 1999-06-30

    The Japanese Society of Applied Glycoscience

References:  18

Cited by:  12

Codes

  • NII Article ID (NAID)
    10008258828
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13403494
  • NDL Article ID
    4785708
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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