CGTaseのアミロース環状化反応  [in Japanese] Cyclization Reaction of CGTase on Amylose  [in Japanese]

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Author(s)

Abstract

The Cyclization reaction of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) from alkalophilic Bacillus sp. A2-5a, Bacillus macerans, and Bacillus stearothermophilus on amylose was reinvestigated by the use of high-performance anion exchange chromatography . When CGTase from alkalophilic Bacillus sp. A2-5a (A2-5a CGTase) was incubated with synthetic amylose, cyclic α-1, 4-glucans with degree of polymerization (DP) from 6 to more than 60 were produced in the initial stage of the reaction. The molecular mass and the cyclic structure of these glucans were determined by TOF-MS and <SUP>13</SUP>C NMR. The larger cyclic α-1, 4-glucans produced in the initial stage of the reaction of A2-5a CGTase were subsequently converted into smaller cyclic α-1, 4-glucans and into the final major product, α-CD. CGTase from B. macerans (B, macerans CGTase) also produced larger cyclic α-1, 4-glucans, which were then converted into smaller cyclic α-1, 4-glucans and into the final major product α-CD. B. macerans CGTase, however, converted larger cyclic α-1, 4-glucans into smaller ones more slowly than A2-5a CGTase did. On the other hand, most of the α-1, 4-glucans produced by the action of B. stearothermophilus CGTase were α-, β-, and β-CD, and only a few larger cyclic α-1, 4-glucans were detected. From these results, a new model of the cyclization reaction of CGTase was proposed .

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 46(2), 207-215, 1999-06-30

    The Japanese Society of Applied Glycoscience

References:  23

Codes

  • NII Article ID (NAID)
    10008259044
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    13403494
  • NDL Article ID
    4785930
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  NDL  J-STAGE 
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