書誌事項
- タイトル別名
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- Cyclization Reaction of CGTase on Amylose.
- CGTase ノ アミロース カンジョウカ ハンノウ
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抄録
The Cyclization reaction of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) from alkalophilic Bacillus sp. A2-5a, Bacillus macerans, and Bacillus stearothermophilus on amylose was reinvestigated by the use of high-performance anion exchange chromatography . When CGTase from alkalophilic Bacillus sp. A2-5a (A2-5a CGTase) was incubated with synthetic amylose, cyclic α-1, 4-glucans with degree of polymerization (DP) from 6 to more than 60 were produced in the initial stage of the reaction. The molecular mass and the cyclic structure of these glucans were determined by TOF-MS and 13C NMR. The larger cyclic α-1, 4-glucans produced in the initial stage of the reaction of A2-5a CGTase were subsequently converted into smaller cyclic α-1, 4-glucans and into the final major product, α-CD. CGTase from B. macerans (B, macerans CGTase) also produced larger cyclic α-1, 4-glucans, which were then converted into smaller cyclic α-1, 4-glucans and into the final major product α-CD. B. macerans CGTase, however, converted larger cyclic α-1, 4-glucans into smaller ones more slowly than A2-5a CGTase did. On the other hand, most of the α-1, 4-glucans produced by the action of B. stearothermophilus CGTase were α-, β-, and β-CD, and only a few larger cyclic α-1, 4-glucans were detected. From these results, a new model of the cyclization reaction of CGTase was proposed .
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 46 (2), 207-215, 1999
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206292267648
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- NII論文ID
- 10008259044
- 10008252669
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- NII書誌ID
- AN10453916
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- ISSN
- 18807291
- 13447882
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- NDL書誌ID
- 4785930
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可