アミノ糖資源の利用に関する新規な試み : キチン脱アセチル化酵素の活用  [in Japanese] New Strategy for Utilization of Amino Sugar Resources : Application of Chitin Deacetylase  [in Japanese]

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Author(s)

    • 徳安 健 TOKUYASU Ken
    • 農林水産省食品総合研究所 National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
    • 林 清 HAYASHI Kiyoshi
    • 農林水産省食品総合研究所 National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
    • 森 隆 MORI Yutaka
    • 農林水産省食品総合研究所 National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

Abstract

Herein we propose a new strategy for utilizing amino sugar resources that involves the enzymatic removal of N-acetyl groups from the amino sugar structures instead of hydrolysis of the sugar chains . We chose chitin deacetylase from a Deuteromycete, Colletotrichum lindemuthianum, as a tool for enzymatic deacetylation of amino sugars. We have purified the enzyme from a culture filtrate to electrophoretic homogeneity (944-fold with a recovery of 4.05%). The optimum temperature of the enzyme was 60t, and the optimum pH was 11.5-12.0 when glycol chitin was used as substrate . The enzyme retained 96% of its activity in the presence of 100 mM sodium acetate . The enzyme was active toward chitin oligomers whose degree of polymerization are more than two, and toward partially N-deacetylated water-soluble chitin. The enzyme could convert (GlcNAc) 36 into fully deacetylated corresponding chitosan oligomers. Conversely, (GlcNAc) 2 was partially deacetylated into 2-acetamido-4-0- (2-amino-2-deoxy-β-D-glucopyranosyl) -2-deoxy-D-glucose [GlcNGlcNAc]. The enzymatic deacetylation method has advantageous characteristics over chemical methods: (1) It never causes unexpected side reactions; (2) It is highly reproducible; (3) Unique compounds such as GlcNGlcNAc can be produced. These basic data on characterization of the enzyme will give us important information for its utilization in glycotechnology.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 46(2), 227-232, 1999-06-30

    The Japanese Society of Applied Glycoscience

References:  23

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008259086
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    13403494
  • NDL Article ID
    4785941
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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