サイクロデキストリン合成酵素の潜在性反応によるa-1,4分岐プルランの生成

• 小林 幹彦

  現勤務先:秋田県総合食品研究所

• 都築 和香子

  農林水産省食品総合研究所

• 舟根 和美

  農林水産省食品総合研究所

• 加藤 陽治

  弘前大学教育学部

書誌事項

タイトル別名

• .ALPHA.-1,4-Branched Pullulans Produced by the Action of Cyclodextrin Glucanotransferase.
• α-1,4-Branched Pullulans Produced by the Action of Cyclodextrin Glucanotransferase
• アルファ 1 4 Branched Pullulans Produced by the Action of Cyclodextrin Glucanotransferase

抄録

The action of cyclodextrin glucanotransferase (CGTase: EC 2.4.1.19) on pullulan was examined. A poor susceptibility of CGTase to pullulan was enhanced by increasing the substrate and enzyme concentrations. In spite of a small increase in reducing sugar, large changes in the molecular size of pullulan were observed especially in high-molecular weight pullulan PF30 (Mw 283, 000). These results indicated that a disproportionation activity of CGTase worked predominantly for the reconstruction of pullulan. The product pullulans showed different responses from pullulan when the interaction with the fluorescent reagent and hydrolysis with isoamylase and glucoamylase were compared. Structural analyses of product pullulans by two-dimensional NMR, HMQC, and methylation indicated that CGTase introduced a-1, 4-branch points at the nonreducing side of maltotriosyl units in pullulan molecule yielding, just like α-1, 6-branch points on the linear back bones of α-1, 4-linkages.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 46 (3), 273-280, 1999

  日本応用糖質科学会

詳細情報

• CRID: 1390001206293341056
• NII論文ID: 10008259222
• NII書誌ID: AN10453916
• ISSN: 13403494
• ISSN: 18807291
• ISSN: 13447882
• DOI: 10.5458/jag.46.273
• NDL書誌ID: 4846254
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010592328
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4846254
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/46/3/46_3_273/_pdf
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可